Community profiling. The application of next generation sequencing technologies to PCR-amplified conserved marker genes to profile microbial communities. This technique allows samples to be probed in great depth (typically able to detect populations representing 0.01% of the community) and also multiple samples to be profiled in parallel.
Metagenomics. Random shotgun sequencing and analysis of bulk DNAs extracted from environmental or clinical samples.
Single cell genomes. Sequencing, assembly and analysis of genomes from uncultured (candidatus) organisms. Cells can be individually sorted by flow cytometry, lyzed, whole genome amplified, normalized and sequenced. Normalization permits more even and higher average coverage and improves assembly metrics.
Metatranscriptomics. Random shotgun sequencing and analysis of cDNAs synthesized from RNAs extracted from environmental or clinical samples. Bulk RNAs are typically depleted in rRNAs prior to sequencing.