Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.

Title	Dielectrophoresis-based discrimination of bacteria at the strain level based on their surface properties.
Publication Type	Journal Article
Year of Publication	2013
Authors	Braff WA, Willner D, Hugenholtz P, Rabaey K, Buie CR
Journal	PloS one
Volume	8
Pagination	e76751
Date Published	2013
ISSN	1932-6203
Abstract	Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research.
DOI	10.1371/journal.pone.0076751

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